Archive for the ‘Disorders & use of Impact R in Research’ Category

Impact-R and Inherited Combined Deficiency of Vitamin K Dependent Clotting Factors

Thursday, March 1st, 2012

Impact-R, a cone and plate analyzer may be of benefit in the monitoring of treatment of VKCFD or Vitamin K Dependent Clotting Factor Deficiency.

VKCFD is a rare autosomal recessive deficiency of Vitamin K dependent factors. This means a deficiency of the clotting factors II, VII, IX and X. This disorder was first described in a female infant who exhibited significant bleeding during the first week of life.

Vitamin K.

Vitamin K dependent factors require ?-carboxylation of glutamic acid residues at their Gla domains to enable binding of calcium to phospholipid membranes. This reaction is catalyzed by an enzyme, ?-glutamyl carboxylase. This enzyme requires reduced Vitamin K as a cofactor.

VKCFD may be difficult to diagnose in newborns because of the routine administration of Vitamin K at birth. VKCFD may be treated by oral or parenteral Vitamin K but there may be cases where Vitamin K does not improve symptoms and factor replacement may be necessary.

Impact-R to Determine Effect of Erythrocytosis and Thrombocytosis

Wednesday, January 18th, 2012

Impact-R, a cone and platelet analyzer can be used to determine the effect of increased erythrocyte and thrombocyte counts on platelet adhesion and aggregation.

In a study by Peerschke, et.al., the cone and platelet analyzer was used to determine platelet adhesion in terms of surface coverage (%SC) and platelet aggregation in terms of aggregate size (AS in um).

A blood clot on electron microscopy.

The study noted that erythrocytosis increased aggregate size and thrombocytosis increased surface coverage. The specimen used was whole blood from healthy individuals with varying RBC and platelet counts.

The study also investigated blood from individuals with myeloproliferative diseases. They noted that differences in the platelet function parameters were seen in MPD patients undergoing different therapies.

Impact-R and Disseminated Intravascular Coagulation

Thursday, January 12th, 2012

Impact-R, a cone and plate analyzer can detect defects in platelet adhesion and aggregation. It can be of use in research about DIC.

What is Disseminated Intravascular Coagulation?

DIC is pathological activation of coagulation mechanisms in response to a variety of substances seen in disease states. Activation of coagulation causes blood clots to form in the small blood vessels of most organs in the body. This has two effects: disruption of blood flow to the organs whose blood vessels have small blood clots resulting in malfunction of the organ; and consumption of blood clotting factors and platelets resulting in bleeding elsewhere in the body.

Normally, the human body is maintained in homeostasis, a balance of coagulation and fibrinolysis, with both processes counterchecking each other through feedback mechanisms. In DIC, these mechanisms are dysregulated. A critical component in the triggering mechanism is tissue factor or TF, also known as platelet tissue factor or Factor III. It is found in subendothelial tissue, platelets and leukocytes. TF is released in response to cytokines, tumor necrosis factor, and endotoxin.

DIC can be caused by many conditions. These include malignancies, obstetrics, trauma, infections, toxins and poisons. These conditions trigger the release of tissue factor and subsequently activate the extrinsic pathway of coagulation.

The role of Impact-R in Research

Since tissue factor can be found in platelets, addition of substances that release tissue factor to platelets will result in coagulation. Impact-R can be used to determine which substances can elicit release of tissue factor.

Impact-R in the Diagnosis of d-SPD

Monday, January 9th, 2012

Impact-R is a cone and plate analyzer which tests platelet function under arterial flow conditions. It can detect defects in platelet adhesion, extension and aggregation such as can be seen in platelet storage pool diseases like a-SPD and s-SPD.

What is d-SPD?

Platelet.

Storage pool disease involving the dense granules was first described in 1972. On Wright-stained smears, the platelets are morphologically normal but are shown to lack d-granules on EM (electron microscopy).  Since these granules are the storage sites for serotonin, ADP and ATP, their lack also means a lack of the said substances.  ADP and ATP are enhancers of platelet aggregation by activation of more platelets which in turn release their dense granule substances and recruit more platelets. Low levels of platelet ADP, ATP result in bleeding diathesis. Examples of bleeding are: easy bruising, epistaxis, post-surgical bleeding, heavy menstrual bleed.

Use of Impact-R in the Diagnosis of d-SPD

Recent studies of platelet adhesion under high-shear stress have been done on patients with d-SPD. One of these is the Impact-R machine. They show a reduced secondary wave of aggregation when stimulated by ADP, epinephrine or thrombin.

Impact-R as Adjunct Diagnostic Test for Congenital Protein C or S Deficiency

Saturday, January 7th, 2012

Impact-R, a test for platelet function, has potential use as adjunctive diagnostic test to identify protein C or S deficiency.

A blood clot.

What is protein C or S deficiency?

Protein C and S are proteins found in the plasma that participate in the coagulation cascade as regulators of thrombin formation by inhibition of Factor VIIIa and Factor Va. Protein C is activated by thrombin-thrombomodulin complex then combines with free Protein S, its cofactor, to inhibit Factor VIIIa and Factor Va.

Inherited protein C or S deficiency is rare and occurs independently of each other. Protein C or S abnormalities include decreased levels of either protein, inability to bind with each other, protein C/S complex that that cannot degrade factors VIIIa and Va, increased clearance of Protein S.

Deficiencies of either protein C or S results in non-inhibition of factors VIIIa and Va and continuous, unchecked formation of thrombin. This leads to hypercoagulability of patient’s blood, and may result in deep vein thrombosis, venous thromboembolism, purpura fulminans or disseminated intravascular coagulation.

What are the tests for protein C or S deficiency?

Laboratory tests include Functional Protein C, Functional Protein S, Protein C Antigen and Protein S Antigen. These are specific for the Protein deificiency.

Adjunctive tests include Bleeding time, Partial Thromboplastin time, Prothrombin time and Thrombin time.

What is the use of Impact-R in diagnosing protein C or S deficiency?

Impact-R can be of use in the diagnosis of protein C or S by determining the effect of adding free protein S and activated protein C in the platelet adhesion and aggregation of whole blood of patients with the suspected deficiency. This can be an alternative in cases where the Protein C or S assays are not available.

Impact-R as Screening Test for Hemophilia A

Thursday, January 5th, 2012

Impact-R, a cone and plate analyzer for platelet adhesion and platelet aggregation may be utilised as a screening test for bleeding disorders such as Hemophilia A.

What is Hemophilia A?

Hemophilia A is a blood disorder that causes prolonged bleeding due to a deficiency of blood clotting Factor VIII.

Hemarthrosis of the elbow joint.

Symptoms are spontaneous bleeding, bruising, blood in the urine, gastrointestinal bleeding, epistaxis, prolonged bleeding from cuts and following surgeries such as circumcision and dental extraction, hemarthroses or bleeding into joints such as the knee. Symptoms may not be apparent in infants but will soon show when the baby starts to crawl or walk. Severity of the symptoms may vary. Major bleeding could be fatal such as those that happen if the patient is involved in a motor vehicular accident or sports-related injuries.

Hemophilia A is a hereditary disorder that is X-linked recessive. The disease manifests only in males because of their single X chromosome, whereas, females have two X-chromosomes. Very rarely, females manifest the disease if both X chromosomes are affected. This happens when the father is a haemophiliac and the mother is a carrier. Affected males mated to non-carriers will have male offspring that do not have the disease and female offspring that are carriers. Carrier females mated to un-affected males will have 50% chance of having male children with the disease and 50% chance of having female children who will be carriers.

The Use of Impact-R

Impact-R could be of use as a bedside screening test in children whose mothers have male relatives that have Hemophilia. It is a simple and rapid test that can determine defects in coagulation affecting platelet function. Studies have to be done, however, to determine the positive and negative predictive value of Impact-R as a screening test for Hemophilia A.

Impact-R as Rapid Screening Test for Acute TTP

Wednesday, January 4th, 2012

The Impact-R machine provides high-shear stress conditions needed by ADAMTS-13 to cleave ULVWF multimers seen in Thrombotic Thombocytopenic Purpura.

Skin lesions in TTP.

Thrombotic thrombocytopenic purpura is a rare disorder of the coagulation cascade. The etiology of TTP includes that of inhibition of function or the deficiency of ADAMTS-13, a metalloprotease enzyme that cleaves von Willebrand factor multimers in high shear stress conditions. If it is inhibited or deficient, the vWF forms ultra large multimers that predispose to thrombi formation in the microvasculature by spontaneously attaching to the platelet membrane GpIba.

A study by Jing Fei Dong, et.al.demonstrates this phenomenon. In the study,

Beads-on-a-string formation of platelets and ULVWF multimers.

cells with GpIba receptors in their membranes and platelets formed long beads-on-a-string formations on the endothelial culture cells when exposed to ultra large von Willebrand factor (ULVWF) multimers under high shear stress conditions.

When blood from a patient with TTP is tested using the Impact-R, surface coverage and aggregate size are expected to be large because of the presence of ULVWF in the patient’s plasma. When the same blood is exposed to plasma from a normal patient (which contains ADAMTS-13), and then tested by the Impact-R, theoretically, there will be a lessening of the surface coverage and aggregate size. This is the subject of a study by Shenkman in which he proposed that Impact-R could be used as a rapid screening test for TTP.

Impact-R and Heyde Syndrome

Thursday, December 29th, 2011

This is a case of a 79-year old man with severe aortic stenosis and recurrent GI bleeding. This case study by Schmid, et.al. proposes that Impact-R to determine platelet function may be more time- and cost-effective compared to electrophoretic analysis of vWF multimers.

Severe aortic stenosis with bleeding diathesis is also known as Heyde syndrome. The pathophysiology: loss of high molecular weight vWF multimers in high shear stress conditions leads to a platelet type von Willebrand disease (acquired vWD, vWD type 2B) which can explain the bleeding tendencies of a patient with severe aortic stenosis.

 

Impact-R in Evaluation of Platelet Reactivity in Metabolic Syndrome

Thursday, December 29th, 2011

Along with other laboratory equipment, the Impact-R has been used in a study by Vaduqanathan, et.al. to evaluate platelet reactivity in patients with metabolic syndrome.

Metabolic syndrome is a combination of several medical conditions that increase the risk for developing cardiovascular disease and diabetes mellitus. It is also known as Syndrome X, insulin resistance syndrome, and Reaven’s syndrome.

The diagnostic criteria for metabolic syndrome (as defined by International Diabetes Foundation) includes central obesity with any two of the following:

-          Raised triglycerides

-          Reduced HDL cholesterol

-          Raised blood pressure

-          Raised fasting plasma glucose

Metabolic syndrome is associated with a prothrombotic state and platelet reactivity can therefore be considered a result. The study by Vaduqanathan, et.al. evaluated the platelet reactivity of patients with metabolic syndrome and their response to aspirin. They concluded that metabolic syndrome increases baseline platelet reactivity and lowers platelet response to aspirin.

Possible Uses of Impact-R in Chediak-Higashi Syndrome Patients

Sunday, December 25th, 2011

Chediak-Higashi syndrome can also be found in killer whales.

Chediak-Higashi syndrome (CHS) is a rare autosomal recessive mutation in the LYST gene. This results in microtubule defects in the cell that causes decreased phagocytosis. The decreased phagocytic activity of the cells in the body leads to recurrent pyogenic infections, skin pigmentation disorders and peripheral neuropathy. Manifestations include neutropenia, oculocutaneous albinism, periodontal disease, and bleeding disorders.

The bleeding disorders are heterogeneous. A study of 8 CHS patients by Al-Sheyyab, et.al. pointed out several mechanisms for the bleeding diathesis in CHS. In one patient, there is abnormal aggregation due to storage pool deficiency (SPD) of ADP and serotonin. In another patient, the aggregation defect resembles that of von Willebrand disease type IIb. Other defects involved the dense bodies and the alpha-granules as well as the platelet membrane.

An avenue of research in the pathophysiology of CHS is therefore opened up by these findings. What are the different platelet defects associated with CHS? What are the different mechanisms leading to an abnormality in platelet aggregation? What is the effect of ristocetin in platelet aggregation and how is this significant in CHS patients. The use of Impact-R will greatly aid the researcher in the investigation of the abovementioned issues.

Calendar
December 2017
M T W T F S S
« Mar    
 123
45678910
11121314151617
18192021222324
25262728293031